2021 Fiscal Year Final Research Report
Finding molecular pathways specific in the highly invasive radioresistant cancer cells and proposing the way to block their invasiveness
| Project/Area Number |
19K08111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52040:Radiological sciences-related
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| Research Institution | National Institutes for Quantum Science and Technology |
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Principal Investigator |
Fujita Mayumi 国立研究開発法人量子科学技術研究開発機構, 量子生命科学研究所, 主幹研究員 (80580331)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 癌細胞の浸潤 / 癌細胞の転移 / 放射線抵抗性 |
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| Outline of Final Research Achievements |
We have recently reported that radiation is effective in reducing the invasion of many cancer cell lines, however, we also confirmed that a minority of cell lines display enhanced invasion following irradiation, including PANC-1. In the case of PANC-1, there were the distinct invading population (INV) within the whole cultured PANC-1 population, which showed resistant to radiation as well as higher metastatic potential, indicated that INV are risk population for cancer treatment. Thus, in this study, we used 7 breast cancer cell lines to further investigate the existence of INV in other cell lines. We found that MDA-MB-468 and SUM149 also include INV population within whole cell population, but of note, their INV were not radio-resistance, indicated that these INV could be killed with radiation. Moreover, we revealed that INV cells have unique character exhibiting hyper-glycolytic phenotype, and thus, inhibitor for glycolysis was effective in blocking their aggressive invasiveness.
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| Free Research Field |
放射線生物学、分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
放射線癌治療をさらに発展させる為には、治療後の再発や浸潤、転移をいかに抑制できるかが重要である。我々はPANC-1を用いた研究から、細胞全体の集団の中には一部、顕著に浸潤能が高い「高浸潤細胞」の集団が存在することを明らかにしてきた。さらに細胞株によっては、それら浸潤細胞は放射線にも抵抗性を示すことも見出した。本研究では、このような治療においてリスクとなり得る「高浸潤細胞」で共通する重要因子を調べ、これら細胞集団を効率良く抑制する方法を提案した。この成果は、癌の浸潤・転移抑制剤の開発や、治療後の診断マーカーの開発にも役立つ成果であり、学術的意義や社会的意義が非常に高い結果であると考えている。
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