Identification of bacteriological factors in neonatal chronic lung disease and establishment of prevention methods

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K08248
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 52050:Embryonic medicine and pediatrics-related
• Research Institution: University of Toyama
• Principal Investigator: Yoshida Taketoshi 富山大学, 学術研究部医学系, 教授 (90361948)
• Co-Investigator(Kenkyū-buntansha): 仁井見 英樹 富山大学, 学術研究部医学系, 准教授 (50401865)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 子宮内感染 / 慢性肺疾患 / 早産児

Outline of Final Research Achievements

In this study, we clarified the following using a highly sensitive PCR method developed at our hospital.
Ureaplasma mycoplasma was the most common causative agent of amniotic fluid infection in preterm infants. Cord blood IL-6, fetal blood WBC, and CRP increased in proportion to the increase in bacterial load. There was no correlation between the incidence of intraventricular hemorrhage, sepsis, RDS, etc. and the bacterial load. Histological chorioamnionitis and funisitis were higher when the bacterial load was high. No correlation was found between the amount of causative bacteria in amniotic fluid and CLD.

Free Research Field

新生児学

Academic Significance and Societal Importance of the Research Achievements

本研究では、当院で開発した高感度PCR法を使用して以下のことを明らかにした。
早産児ではウレアプラズマ・マイコプラズマが最も羊水感染の原因菌として多かった。菌量が多くなることに比例して、臍帯血IL-6,児血WBC、CRPが増加した。脳室内出血、敗血症、RDSなどの発症率と菌量との間に相関はなかった。菌量が多いと組織学的絨毛膜羊膜炎と臍帯炎が高率であった。羊水中の原因菌の菌量とCLDとはまったく相関関係を認めなかった。