2022 Fiscal Year Final Research Report
Establishment of a risk evaluation system for spina bifida using spinal cord primordium derived from human induced pluripotent stem cells
Project/Area Number |
19K08342
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
|
Research Institution | Nagoya City University |
Principal Investigator |
Ninomiya Hiromasa 名古屋市立大学, 医薬学総合研究院(医学), 講師 (40514237)
|
Project Period (FY) |
2019-04-01 – 2023-03-31
|
Keywords | 形態形成 / 細胞運動 / 組織伸長 |
Outline of Final Research Achievements |
We established an in vitro system for tissue elongation, investigated the mechanism of elongated tissue morphogenesis, and attempted to reproduce malformations in vitro. First, we proved that tissue elongation occurs in the anterior-posterior axial direction only when the anterior and posterior tissues are juxtaposed position in vitro, and confirmed that the cell differentiation pattern of this tissue is similar to that of normal embryos. Next, convergent extension, not mitosis, is demonstrated as the main driving force of tissue elongation by the analysis of cell behavior during elongation in vitro. Furthermore, cell movements-related factors were shown to localize directionally in elongating tissues. Finally, treatment with teratogens or knockdown of cell movements-related genes have suppressed tissue elongation in vitro.
|
Free Research Field |
発生生物学
|
Academic Significance and Societal Importance of the Research Achievements |
本件究では正常胚の組織伸長過程を再現する試験管内系を作った。系は細胞種を限定した条件で形態形成を再現するので解析が容易であり、通常の細胞培養施設で再現可能であることから、専門外の多くの研究者にも組織伸長の解析を試みる機会を提供する。我々が系を用いて組織伸長の仕組みを調べた結果、組織の方向性、および伸長運動関連因子の局在が伸長に必要な条件であることが明らかになった。さらに系を用いて奇形発症を再現することにも成功したので、本研究で作った系を用いた解析は、モデル動物やヒト疫学研究で得られた知見との相乗効果で、奇形発症の原因解明・予防法開発に進歩をもたらすことが期待される。
|