2022 Fiscal Year Final Research Report

Efficacy of mesenchymal stem cell for prevention of esophageal stricture after endoscopic submucosal dissection

Research Project

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Project/Area Number	19K08390
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 53010:Gastroenterology-related
Research Institution	Niigata University
Principal Investigator	Hashimoto Satoru 新潟大学, 医歯学総合研究科, 客員研究員 (10768667)
Co-Investigator(Kenkyū-buntansha)	寺井崇二新潟大学, 医歯学系, 教授 (00332809) 上村顕也新潟大学, 医歯学総合病院, 講師 (00579146) 佐藤裕樹新潟大学, 医歯学系, 助教 (50644556) 土屋淳紀新潟大学, 医歯学総合病院, 講師 (70464005) 横尾健新潟大学, 医歯学総合研究科, 特任准教授 (80750629)
Project Period (FY)	2019-04-01 – 2023-03-31
Keywords	食道狭窄 / 間葉系幹細胞 / 皮膚瘻孔モデル / 線維化 / 内視鏡的粘膜下層剥離術
Outline of Final Research Achievements	Esophageal stricture following endoscopic treatment for esophageal cancer is caused by fibrosis in the submucosal layer during the mucosal healing process. In this study, we created a rat skin fistula model and investigated the effects of mesenchymal stem cells (MSCs), which are known to have anti-inflammatory and anti-fibrotic properties, by injecting them. In the MSC local injection group, we observed accumulation of macrophages coinciding with the administration site, and a significant presence of CD31-positive cells suggesting neovascularization in the vicinity. Conversely, these reactions were not observed in the MSC non-local injection group, leading us to speculate that local injection of MSCs induces the initiation of tissue healing.
Free Research Field	消化器内科
Academic Significance and Societal Importance of the Research Achievements	MSCの局注および現在行っているMSC徐放化シートの効果が明らかになれば、イヌ食道粘膜欠損モデルにて検証を行う。イヌ食道粘膜欠損モデルは当科で作成可能であり、MSCの効果が確認できれば、内視鏡切除後の食道狭窄予防における臨床応用の段階となる。