2021 Fiscal Year Final Research Report
Mechanisms of extrahepatic complications in NAFLD/NASH with focus on exosomes
Project/Area Number |
19K08417
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 53010:Gastroenterology-related
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Research Institution | Niigata University |
Principal Investigator |
Takamura Masaaki 新潟大学, 医歯学総合研究科, 客員研究員 (20422602)
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Co-Investigator(Kenkyū-buntansha) |
横尾 健 新潟大学, 医歯学総合研究科, 特任准教授 (80750629)
寺井 崇二 新潟大学, 医歯学系, 教授 (00332809)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | エクソソーム / 非アルコール性脂肪肝疾患 / 非アルコール性脂肪肝炎 / 肝外合併症 / プロテオーム解析 |
Outline of Final Research Achievements |
In this study, we observed the dynamics of exosomes in human CD63-GFP Tg rats, which can visualize exosomes, and conducted a proteome analysis of exosome-incorporated proteins in the blood of rats fed a high-fat diet and control rats to search for candidate proteins involved in the pathogenesis of extrahepatic complications. We searched for candidate proteins involved in the pathogenesis of extrahepatic complications. Exosomes were identified in the stomach and kidney, but their expression in the liver was insufficient for further validation. No protein was identified that was expressed in both groups with a 2-fold or greater difference in expression. Many of the proteins identified in high-fat diet-fed NAFLD rats alone have not been reported in NAFLD and require further investigation in the context of clinical data.
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Free Research Field |
消化器内科学
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Academic Significance and Societal Importance of the Research Achievements |
今回の研究で、エクソソーム内タンパク質のプロテオーム解析から多くの候補タンパク質が得られた。これらの今後の解析により、非アルコール性脂肪肝疾患(NAFLD)/非アルコール性脂肪肝炎(NASH)の肝外合併症発症メカニズムの分子・細胞レベルでの解明がされ創薬につながれば、わが国でも1000万人以上いると推定されている本疾患の生命予後の改善に大いに役立つと考える。
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