Elucidation of the mechanism of hepatocarcinoma progression by glycosylation

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K08422
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Okayama University
• Principal Investigator: Onishi Hideki 岡山大学, 大学病院, 講師 (30595468)
• Co-Investigator(Kenkyū-buntansha): 能祖 一裕 岡山大学, 医学部, 客員研究員 (10314668) ; 白羽 英則 岡山大学, 大学病院, 講師 (40379748)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: アガラクトシル化

Outline of Final Research Achievements

Six types of IgG-binding glycans were identified in the sera of patients with hepatocellular carcinoma and healthy subjects with a combined background. Among them, galactose binding glycans were found to be decreased in patients with hepatocellular carcinoma. While searching for the cause, we confirmed that galactosyltransferase (B4GALT3) in lymphocytes was decreased by adding culture supernatant of a hepatocellular carcinoma cell line (Huh7). Since miR was suspected to be the responsible substance in the culture supernatant by serum fractionation, we narrowed down the target to miR. As a result, 308 miRs were detected in the culture supernatant of Huh7 cells, among which miR27b-3p, which binds to B4GALT3, was found to downregulate B4GALT3 expression in the lymphocytes.

Free Research Field

医歯薬学

Academic Significance and Societal Importance of the Research Achievements

糖鎖変化を起こさせる物質を同定するとともに、肝細胞癌病態への関与を明らかとすることは、新たな診断法や治療標的の解明にもつながる。また、免疫グロブリンのガラクトースの変化は癌種を超えた現象であるため、社会的波及効果が極めて高い。