2021 Fiscal Year Annual Research Report
Cytoglobin overexpression inhibits liver fibrosis and cancer development via anti-oxidant function
| Project/Area Number |
19K08428
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| Research Institution | Osaka City University |
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Principal Investigator |
LE THITHANHTHUY 大阪市立大学, 大学院医学研究科, 特任講師 (10572175)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | Cytoglobin / Hepatic stellate cells / Anti-fibrosis / Liver cancer / Antioxidant |
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| Outline of Annual Research Achievements |
We have examined therapeutic effects of 6-His tagged recombinant human Cytoglobin (rhCYGB) protein against mouse liver injuries/fibrosis and human HSC activation. We have completed investigating the role of endogenous CYGB on liver diseases using CYGB deficient (Cygb-KO) mice and CYGB transgenic (Cygb-TG) mice. We have completed 3 models of liver injuries using wild-type (WT), Cygb transgenic (TG), and knockout (KO) mice. Mice were sacrificed at 10 weeks after thioacetamide (TAA) injection, 16 weeks after choline deficient amino acid define diet (CDAA) feeding plus 4 weeks recovery or 12 months after diethylnitrosamine (DEN) treatment. These all three models showed that despite of different etiologies, absence of Cygb significantly exacerbated liver damage, fibrosis and reactive oxygen species (ROS) formation. All these manifestations were attenuated in Cygb-overexpressing mice. We have further produced 6His-tagged recombinant human CYGB (His-CYGB), traced its bio-distribution in vitro, and assessed its function in suppressing fibrosis synthesis in cultured human hepatic stellate cells (HHSteCs). We then compare the effect of full-length of rhCYGB protein and its shortening peptides. We found that the effect of rhCYGB in inhibiting Collagen synthesis in vitro can be kept with its core region from acid amine residue 18 to 180 in comparison with its full-length from 1-190 acid amine.
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[Journal Article] Cytoglobin Attenuated Pancreatic Cancer Growth in vitro and in vivo via Scavenging Function for Reactive Oxygen Species2022
Author(s)
3.Dinh VH*, Thuy LTT*, Hai H, Hieu VN, Kimura K, Oikawa D, Ikura Y, Ninh QD, Hoang TH, Sato-Matsubara M, Dong MP, Hanh NV, Uchida-Kobayashi S, Tokunaga F, Kubo S, Ohtani N, Yoishizato K, and Kawada N
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Journal Title
Oncogenesis
Volume: 0-0
Pages: 0-0
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Role of CD40 in Hepatocellular Carcinoma2021
Author(s)
Ngo Vinh Hanh, Le Thi Thanh Thuy, Hoang Hai, Vu Ngoc Hieu, Sawako Uchida-Kobayashi, Masaru Enomoto, Akihiro Tamori, and Norifumi Kawada
Organizer
The Asian Pacific Association for the Study of the Liver Single Topic Conference (APASL STC)
Int'l Joint Research
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[Presentation] Cytoglobin impairs pancreatic cancer growth in vitro and in vivo2021
Author(s)
Dinh Viet Hoang, Le Thi Thanh Thuy, Hoang Hai, Vu Ngoc Hieu, Ninh Quoc Dat, Truong Huu Hoang, Dong Minh Phuong, Ngo Vinh Hanh, Yoshihiro Ikura, Kenjiro Kimura, and Norifumi Kawada
Organizer
Annual Meeting of the Digestive Disease Week (DDW)
Int'l Joint Research
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[Presentation] Cytoglobin inhibits pancreatic cancer growth in vitro and in vivo2021
Author(s)
Dinh Viet Hoang, Le Thi Thanh Thuy, Hoang Hai, Vu Ngoc Hieu, Ninh Quoc Dat, Truong Huu Hoang, Dong Minh Phuong, Ngo Vinh Hanh, Yoshihiro Ikura, Kenjiro Kimura, and Norifumi Kawada
Organizer
The 107th General Meeting of the Japanese Society of Gastroenterology
