2023 Fiscal Year Final Research Report
Establishment of disease models and elucidation of the pathogenesis of inflammatory bowel disease using human iPS cells
| Project/Area Number |
19K08453
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | Osaka Medical and Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
友田 紀一郎 大阪医科薬科大学, 医学部, 非常勤講師 (50362843)
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | 炎症性腸疾患 / iPS細胞 |
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| Outline of Final Research Achievements |
Induced pluripotent stem cells (iPS cells) are expected to be a fundamental technology not only for regenerative medicine but also for elucidating the pathophysiology of diseases and drug discovery research. In this study, we attempted to generate a disease model of inflammatory bowel disease using human iPS cells to provide a new research platform for inflammatory bowel disease, for which there is still no fundamental cure. We induced differentiation of intestinal epithelial cells from healthy human iPS cells into intestinal epithelial cells in the small intestine and generated an intestinal inflammatory model in vitro. We also suppressed ATG16L1, a susceptibility gene for inflammatory bowel disease, and elucidated part of the pathological mechanism by which autophagy is involved in intestinal inflammation.
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| Free Research Field |
inflammatory bowel disease
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| Academic Significance and Societal Importance of the Research Achievements |
炎症性腸疾患の研究手法としては一般的に、マウス等による動物実験や、ヒトの腸管組織検体を用いた研究などが行われているが、動物実験では種間の差異が存在し、ヒト組織においては検体採取に限界がある。ヒトiPS細胞から腸管組織へと分化させ、炎症性腸疾患の疾患モデルとして研究に用いることが可能となれば、種間の差異や検体採取の限界といった従来の課題が解決され、今後、創薬研究や病態メカニズムの解明に繋がることが期待される。
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