Epigenome alternations of sarcomeric genes in human dilated cardiomyopathy

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08547
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Iwate Medical University
• Principal Investigator: Satoh Mamoru 岩手医科大学, 医歯薬総合研究所, 客員教授 (90305996)
• Co-Investigator(Kenkyū-buntansha): 大桃 秀樹 岩手医科大学, 医歯薬総合研究所, 講師 (90453406)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 拡張型心筋症 / サルコメア遺伝子 / DNAメチル化

Outline of Final Research Achievements

This study has investigated epigenome alternations of sarcomeric genes in human dilated cardiomyopathy (DCM). This study included 50 patients with DCM and 50 control subjects. Genome DNA was taken from endomyocardial biopsy samples obtained from patients wtih DCM and controls. Ten DNA methylation sites of sarcomeric genes were analyzed using PyroMark system. There was no significant differences in DNA methylation between patients wtih DCM and controls. This study has established the methodology of epigenome alternations of sarcomeric genes in human DCM.

Free Research Field

循環器内科

Academic Significance and Societal Importance of the Research Achievements

本研究では、孤発性拡張型心筋症(DCM)でのサルコメア蛋白遺伝子異常、特に、DNAメチル化解析に着目して検討した。10箇所の代表的なサルコメア蛋白遺伝子のDNAメチル化部位について解析したが、DCMに特異的なDNAメチル化部位を同定には至らなかった。しかし、本研究の学術的意義して心筋生検組織を用いたDNAメチル化解析の方法論を確立が挙げられる。