Understanding in spatio-temporal regulation mechanism of thrombus formation and dissolution for realization of appropriate antithrombotic therapy

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08577
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Suzuki Yuko 浜松医科大学, 医学部, 教授 (20345812)
• Co-Investigator(Kenkyū-buntansha): 浦野 哲盟 浜松医科大学, 医学部, 特命研究教授 (50193967) ; 佐野 秀人 浜松医科大学, 医学部, 助教 (80623842) ; 本藏 直樹 浜松医科大学, 医学部, 准教授 (40518081)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 線維素溶解（線溶） / 可視化解析 / 血小板 / 血栓形成 / トロンビン生成 / トロンボモジュリン / トロンビン活性化線溶抑制因子 / 蛍光顕微鏡

Outline of Final Research Achievements

The blood coagulation system is precisely regulated by either facilitating the activation of its cascade, resulting in prompt hemostatic thrombus formation at the vascular injury site, or preventing thrombus formation by inhibiting their activation in the vessels. It is well known that intravascular thrombus forms easily in various pathological conditions such as atherosclerosis, lifestyle-related disease, and aging, including atrium fibrillation. Antithrombotic drugs, either used for prophylactic or treatment in thrombotic patients, suppress hemostatic thrombus formation but increase the risk of bleeding. Therefore, the formation and dissolution of a thrombus should be clearly explained to deal with such a problem. This study investigated the formation and dissolution of a thrombus by directly visualizing the process using fluorescence microscopy. As a result, we expect the possibility of an effective treatment for thrombosis that reduces the risk of bleeding.

Free Research Field

血栓止血学　生理学

Academic Significance and Societal Importance of the Research Achievements

心筋梗塞や脳梗塞をはじめとした従来の血栓性疾患のみならず、近年では新型コロナウイルス感染症の重症化ならびにワクチン接種における血栓症リスクがクローズアップされた。本研究では血小板の活性化、凝固反応の活性化、生じた血栓を溶かす線維素溶解（線溶）の各反応系を一連の時空間的に制御される反応系として解析した。これをさらに各病態に応じた反応系として捉えることで、より適切な血栓症の治療・予防法の確立につながることが期待できる。