Identification of nephritogenic abnormally glycosylated IgA1 and its application to point of care testing

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08691
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Fujita Health University
• Principal Investigator: Takahashi Kazuo 藤田医科大学, 医学部, 教授 (90631391)
• Co-Investigator(Kenkyū-buntansha): 湯澤 由紀夫 藤田医科大学, 医学部, 教授 (00191479) ; 坪井 直毅 藤田医科大学, 医学部, 教授 (50566958)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: IgA腎症 / 糖鎖異常IgA / バイオマーカー / 質量分析 / 糖鎖医学

Outline of Final Research Achievements

We aimed to develop a Point of Care Testing (POCT) by identifying detailed glycan structure of nephritogenic abnormally glycosylated IgA1 increasing specifically in IgA nephropathy (IgAN). We quantitatively profiled glycoforms of serum IgA1 derived from Japanese and Caucasian patients of IgAN and identified candidate glycoforms that are specifically increased in IgAN. We are currently generating monoclonal antibodies to specific glycoform to develop POCT.

Free Research Field

腎臓内科学

Academic Significance and Societal Importance of the Research Achievements

日本人と白人のIgA腎症に共通のIgA1の糖鎖構造の特徴を同定した。新規診断薬の開発のみならず，糖鎖異常IgA1の産生機構の解明，さらには新規治療標的につながることが期待される。