2021 Fiscal Year Final Research Report
Characterization of the intratumoral ecosystem in melanoma by single-cell RNA-seq analysis
Project/Area Number |
19K08752
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 53050:Dermatology-related
|
Research Institution | Sapporo Medical University |
Principal Investigator |
UHARA HISASHI 札幌医科大学, 医学部, 教授 (40201355)
|
Project Period (FY) |
2019-04-01 – 2022-03-31
|
Keywords | シングルセルシークエンス / 悪性黒色腫 / バーコード |
Outline of Final Research Achievements |
The aim of the study is to establish of the method to obtain the single-cell gene expression of RNA in melanoma cells from the resected tumor samples. The method is planned according to the following steps (Hashimoto S, Scientic Reports 7;14225, 2017). In the first case, over expression of MAPK pathway-related molecules and HLA class 1 and lower expression of HLA class 2 and PD-L1 were observed in melanoma cells in 2 of 5 clusters. However, in next 5 examined cases, synthesis of cDNA of a single cell was not stable. We thought that melanin blocked the enzyme activity. We have tried several methods to remove melanin from tissue samples, however it has not been sufficient yet. Now, we test other methods including filters.
|
Free Research Field |
皮膚科学
|
Academic Significance and Societal Importance of the Research Achievements |
腫瘍組織には不均一性が存在するため、組織の塊を対象とした研究では平均化されたデータしか得られず、重要なサブグループの所見が埋もれてしまう可能性がある。本研究では完全に手技の確立に至らなかったが、1例において、腫瘍細胞におけるMAPK、AKT/PI3K関連の細胞増殖関連分子とHLAやPD-L1などの免疫関連分子の発現の関係性を明らかにすることができ、今後の研究の発展につながる糸口は見いだせたと考える。
|