2021 Fiscal Year Final Research Report
Molecular characterisation of invasive infection caused by beta haemolytic Streptococci using the organotypic human skin tissue model
| Project/Area Number |
19K08964
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 54030:Infectious disease medicine-related
|
|---|
| Research Institution | Meijo University (2021)
Tokyo University of Pharmacy and Life Science (2019-2020) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2022-03-31
|
| Keywords | β溶血性レンサ球菌 / 3次元皮膚組織モデル / SDSE |
|---|
| Outline of Final Research Achievements |
Little is known about the mechanism of the onset of Streptococcus dysgalactiae subsp equisimilis (SDSE) infection. One of the reasons for this is because there is no appropriate infection model. In this study, we tried to construct the infection model using a three-dimensional skin tissue culture system. To make the model more accurate, adipocytes were added in the model. Infection from SDSE isolated from an invasive infection caused destruction of the epithelial layer and tissue injury. In addition, we demonstrated that some of the strains formed a biofilm-like structure on the tissue, which could contribute to differences in antimicrobial susceptibility between in vivo and in vitro environments.
|
| Free Research Field |
感染症学、臨床微生物学
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究では、ヒトの培養細胞を用いた3次元皮膚組織モデルを用いて、Streptococcus dysgalactiae subsp equisimilis (SDSE)感染実験系の構築を行った。その結果、本モデルはSDSEによる皮膚障害と組織の損傷を表すことが示された。このモデルを使用すれば、in vitroでSDSEの病態を再現できると考えられ、今後SDSEの詳細な侵襲性メカニズムの解明が期待される。
|
