Evaluation for the maturation of insulin-producing cells differentiated from ADSC by the methylation status

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55010:General surgery and pediatric surgery-related
• Research Institution: The University of Tokushima
• Principal Investigator: IKEMOTO Tetsuya 徳島大学, 病院, 特任教授 (20398019)
• Co-Investigator(Kenkyū-buntansha): 齋藤 裕 徳島大学, 病院, 講師 (50548675) ; 森根 裕二 徳島大学, 大学院医歯薬学研究部(医学域), 准教授 (60398021) ; 黒田 暁生 徳島大学, 先端酵素学研究所, 准教授 (70571412) ; 居村 暁 徳島大学, 大学院医歯薬学研究部(医学域), 徳島大学専門研究員 (90380021)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: Insulin producing cell / methylation status / 成熟度評価 / 再生医療

Outline of Final Research Achievements

We have investigated the quantification of methylation status for evaluating insulin-producing cell (IPC), which were differentiated from adipose-derived stem cell (ADSC) by our established protocol, because we assumed that the methylation status could be used for an index for evaluating IPC maturation by the revelation of its cell lineage (IPC differentiation lineage). We proved that IPC maturation status depended on zinc-ion concentration, and that could be estimated by zinc-ion in the supernatant of the culture medium, and that zinc-ion were corelated to IPC methylation status. Moreover, we found that the methylation status was changed by the mechanical destruction of pancreaticβcells. Thus, the changes of IPC maturation status could be used as an index for IPC quality control during its manufacturing process.

Free Research Field

消化器外科（移植）、再生医療

Academic Significance and Societal Importance of the Research Achievements

我々が研究を進めている脂肪由来幹細胞からのinsulin-producing cell (IPC)分化誘導の臨床応用に際し、ヒトスケール製造においては、そのquality control が絶対条件である。我々の結果はmethylation status によってIPCを評価しうることを示唆するほか、破壊される膵島（β細胞）を予測しうるというものであり、科学的知見とともに実臨床にも意義が大きい。