2021 Fiscal Year Final Research Report

Study of the small intestine reproduction with the bowels epithelium organoid and the bloodstream preservation Biotube

Research Project

PDF

Project/Area Number	19K09067
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 55010:General surgery and pediatric surgery-related
Research Institution	The University of Tokyo
Principal Investigator	Komura Makoto 東京大学, 医学部附属病院, 特任教授 (10422289)
Co-Investigator(Kenkyū-buntansha)	中山泰秀東京大学, 医学部附属病院, 客員研究員 (50250262) 鈴木啓介埼玉医科大学, 医学部, 客員講師 (50724887) 松本有加順天堂大学, 医学部, 助教 (50813672) 藤代準東京大学, 医学部附属病院, 教授 (60528438) 中村哲也順天堂大学, 大学院医学研究科, 特任教授 (70265809) 鈴木完獨協医科大学, 医学部, 准教授 (80598508)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	小腸上皮オルガノイド / biotube / 遊離腸管節
Outline of Final Research Achievements	A technique to culture the cell including the stem cell of the small intestine epithelium as organoid was developed. We performed an intestinal tract reproduction study using encapsulation fiber-related body (Biotube) and small intestine epithelium organoid to encapsulate mold people. ①Disseminated the small intestine epithelium organoids were adhered to internal lumen of biotube in vitro histologically. ②Disseminated the small intestine epithelium organoids were adhered to internal lumen of biotube withI E-Cadher expression in vivo. ③We succeeded in an end-to-end anastomosis of the biotube to vascularized isolation small intestine in the subcutaneous, but there is no the self-replication of the small intestine mucous membrane and the smooth muscle.
Free Research Field	再生医療
Academic Significance and Societal Importance of the Research Achievements	小腸上皮オルガノイドが鋳型被包化組織体(Biotube)に接着することは、新たな知見を得ることができた。また、腸液が流れない状況ではあるが、biotubeと腸管が吻合することが可能であることを確認しており、新たな腸管再生の足場材料を見出すことができた。これらの知見と技術は、短小腸に対する新たな腸管再生手法を示唆する。