Clinical significance of RNA editing marker in patients with CRC and UC-CRC

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Mie University
• Principal Investigator: Okugawa Yoshinaga 三重大学, 医学部附属病院, 教授 (30555545)
• Co-Investigator(Kenkyū-buntansha): 問山 裕二 三重大学, 医学系研究科, 教授 (00422824) ; 大北 喜基 三重大学, 医学部附属病院, 講師 (20378342) ; 楠 正人 三重大学, 医学系研究科, 寄附講座大学教員 (50192026) ; 荒木 俊光 三重大学, 医学系研究科, リサーチアソシエイト (70343217)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 潰瘍性大腸炎 / 大腸癌 / メチル化

Outline of Final Research Achievements

Our study showed that RNA editing was occurred tissue-specific pattern in colorectal cancer (CRC), and series of in vitro analysis revealed that RNA editing was deeply involved in disease development in CRC. In addition, intimate correlation between cancer cells and fibroblast cells via mechanism of RNA editing for CRC development in cancer microenvironments also clarified. In contrast, identified RNA editing cites did not correlated with aging factors in ulcerative colitis (UC) or UC-associated CRC, and further studies are need. Accompanied with this study, we successfully identified novel CpG site in DNA methylation level to detect patients with UC-CRC with high accuracy (AUC:0.84) via assessment of non-cancerous rectal mucosa.

Free Research Field

エピゲノム

Academic Significance and Societal Importance of the Research Achievements

本研究結果により、RNA後天的修飾の新たな大腸癌発癌・癌進展における機序が解明され、またエピゲノム機序を利生することで、あらたな潰瘍性大腸炎関連癌早期診断マーカー開発の一助となる可能性が考えられた。