2021 Fiscal Year Final Research Report
Elucidation of molecular mechanism of maspin associated with poor prognosis of patients with lung squamous cell carcinoma
| Project/Area Number |
19K09304
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55040:Respiratory surgery-related
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
坂部 友彦 鳥取大学, 医学部, 助教 (50639747)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | Maspin / 肺扁平上皮癌 / 細胞内局在 / 細胞間接着 / 細胞浸潤 |
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| Outline of Final Research Achievements |
In this study, we investigate the role of maspin, whose function in tumor cells depend on its subcellular localization, in non-small cell lung cancer (NSCLC). We found that the regulatory mechanism of maspin subcellular localization is disrupted in some cell lines by establishment of NSCLC cell line stably expressing of maspin. Additionally, LK-2-maspin and A549-maspin, which express maspin in the nucleus and cytoplasm, showed decreased cell invasive capability, whereas RERF-LC-KJ-maspin and RERF-LC-AI-maspin, which express maspin in the cytoplasm, showed significantly increased cell invasive capability. Furthermore, we found that decreased expression of N-cadherin is associated with the suppression of cell invasion in A549-maspin.
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| Free Research Field |
病理学
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| Academic Significance and Societal Importance of the Research Achievements |
これまでmaspinは癌抑制遺伝子としての機能が注目されていたが、本研究によって癌に対する機能は細胞内局在に応じて変化し、抑制的に働くだけでなく促進的にも働くという新たな知見を得た。そのため、本研究結果は癌細胞生物学における新たな研究領域の確立に貢献するものであると考えている。また、細胞質に局在するmaspinは、癌細胞の悪性度に対して促進的に働くため、maspin発現を変化させることなく核内への移行を誘導することで抗腫瘍効果を発揮する新たな作用機序を有する新規癌治療薬開発の基盤研究となると考えている。
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