2021 Fiscal Year Final Research Report
Studies on postoperative delirium and expression of neurotransmitter receptors in central nervous system.
| Project/Area Number |
19K09357
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55050:Anesthesiology-related
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉田 圭佑 福島県立医科大学, 医学部, 助手 (00769573)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 術後せん妄 / ドパミン / アセチルコリン / ｍRNA / 麻酔薬 / 鎮静薬 |
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| Outline of Final Research Achievements |
The aim of the present study is to investigate whether several anesthetics affect expression of dopamine D2 and acetylcholine M1 receptors in the rat brain. Thirty-six male rats were divided into six groups (n = 6); five groups for anesthetics and one for control. The five groups were anesthetized with either dexmedetomidine, propofol, midazolam, sevoflurane, or nitrous oxide for 4 hrs. Then, real-time polymerase chain reaction was performed to examine the expression of Drd2 (cord dopamine D2 receptor) and Chrm1 (cord acetylcholine M1 receptor) in the cerebral cortex, hippocampus, corpus striatum, brain stem, and cerebellum. There were no significant differences among the groups regarding Drd2 and Chrm1 mRNA expression of each region of the brain. Postsynaptic changes of dopamine D2 and acetylcholine M1 receptors due to administration of dexmedetomidine, propofol, midazolam, sevoflurane, and nitrous oxide are unlikely to occur at the doses of each anesthetic used in the present study.
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| Free Research Field |
麻酔科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究のプロトコルでは、現在臨床利用されている5種類の麻酔・鎮静薬、デクスメデトミジン、プロポフォール、ミダゾラム、セボフルラン、亜酸化窒素は、ドパミンD2受容体とアセチルコリンM1受容体におけるシナプス後の変化を起こさないことが示唆された。術後せん妄（POD）/術後認知機能障害（POCD）の病態は完全に解明されておらずいくつかの説がある。本研究成果は、POD/POCDの病態が麻酔薬の神経伝達物質受容体に及ぼす影響のみでは説明できないことを支持するものであり、この分野のさらなる研究が必要であることを示した点で学術的・社会的意義がある。
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