Identification of endothelial cell origin microRNA as a clinical marker and therapeutic target of EBI after SAH

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09516
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Keio University
• Principal Investigator: Takahashi Satoshi 慶應義塾大学, 医学部(信濃町), 講師 (20383870)
• Co-Investigator(Kenkyū-buntansha): 荒井 信彦 慶應義塾大学, 医学部(信濃町), 助教 (80793801)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: くも膜下出血 / マイクロRNA / 早期脳障害

Outline of Final Research Achievements

The brain capillary endothelial cell was isolated from the SAH model mouse, and the research was carried out in order to clarify the gene expression change after SAH specialized for the endothelial cell which suffered from EBI, and to identify the microRNA detectable in the peripheral blood which is concerned in the gene expression change in the brain capillary endothelial cell. Hsa-miR-619-5p whose expression was elevated in the peripheral blood of patients with subarachnoid hemorrhage had targeted Gpx8, Soat1, and Rgs16 whose expression was decreased in capillary endothelial cells after subarachnoid hemorrhage, suggesting that hsa-miR-619-5p may be a microRNA that can be used as biomarkers of EBI detectable in the peripheral blood involved in gene expression changes after SAH specialized for brain capillary endothelial cells that gave rise to EBI.

Free Research Field

脳神経外科学

Academic Significance and Societal Importance of the Research Achievements

本研究において同定したhsa-miR-619-5pはこれまで報告がないくも膜下出血後の早期脳障害（EBI）に関するバイオマーカーとして臨床上有用なマイクロRNAである可能性がある。EBIを早期の時点で客観的に捉える指標が確立されれば、EBIおよび続発するDCIに対して備えることが可能になり、壮年期にも発症し、重篤な後遺症を残し得る疾患であるがゆえにその予後改善が社会的にも大きな意義があると期待される疾患であるくも膜下出血の予後改善に寄与し得るものと期待する。