Fundamental study of glycosaminoglycan degradation to reduce thickening of the yellow ligament

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09600
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Shimane University
• Principal Investigator: Tsuchiya Mikako 島根大学, 学術研究院医学・看護学系, 教授 (90188582)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 腰部脊柱管狭窄症 / 黄色靭帯 / グリコサミノグリカン / コンドロイチン硫酸 / ケラタン硫酸

Outline of Final Research Achievements

Biochemical analysis of the thickened yellow ligament of patients with lumbar spinal canal stenosis were performed using mass spectrometry and complete solubilization of the tissue with heat-stable proteolytic enzymes. Glycosaminoglycans, collagen, and elastin were quantified and compared to normal yellow ligament. Among glycosaminoglycans, the monosulfate of chondroitin sulfate and keratan sulfate were found to be increased.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

肥厚黄色靭帯で増加している構成成分がグリコサミノグリカンのうち、コンドロイチン流酸の一硫酸体とケラタン硫酸であることが明らかになったことで、これらの分子が脊椎管狭窄症の治療標的をなり得ることが明らかになった。