2022 Fiscal Year Final Research Report
Effect of difference in fixation methods of tendon graft and the microfracture procedure on tendon-bone junction healing
| Project/Area Number |
19K09626
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 56020:Orthopedics-related
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
齋藤 太一 岡山大学, 医歯薬学域, 助教 (70644384)
|
|---|
| Project Period (FY) |
2019-04-01 – 2023-03-31
|
| Keywords | エンテーシス |
|---|
| Outline of Final Research Achievements |
There are generally two methods of fixation for tendon grafts used in ligament reconstruction: bone tunnel (BT) fixation and anchor fixation. The microfracture (Mf) procedure is a technique to induce bleeding from the bone marrow, and the bleeding may contain cells with differentiation potential. However, few studies have compared the effects of the Mf procedure with those of the fixation methods.
Histological evaluation of the BT + Mf and A + Mf groups showed healing with fibrocartilage formation at the tendon graft-bone junction. The mRNA expression showed significant increase in type 2 collagen, Scleraxis, and SRY-box9 in the BT + Mf and A + Mf groups. In biomechanical tests, the BT + Mf and A + Mf groups showed significantly increased tensile strength compared with the BT － Mf and A － Mf groups. The Mf procedure resulted in fibrocartilage formation at the tendon-bone junction in the BT and anchor fixation and improved the fixation strength at 4 weeks.
|
| Free Research Field |
Orthopedics
|
| Academic Significance and Societal Importance of the Research Achievements |
この研究により移植腱に対するenthesis形成のための骨孔形成の影響や、移植腱の理想的な固定法について大きな発展がもたらされると考えられる。またこの研究により骨孔の形成がenthesisの形成や発達に重要な役割を担うことが明らかになったことで、今後成長因子の補充と手術療法を組み合わせた靭帯再生医療として新たな治療戦略をもたらしうると考えている。
|
