New strategy for the diagnosis of osteonecrosis of the femoral head

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09651
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Hiroshima University
• Principal Investigator: Shoji Takeshi 広島大学, 医系科学研究科(医), 寄附講座助教 (50736569)
• Co-Investigator(Kenkyū-buntansha): 味八木 茂 広島大学, 病院(医), 講師 (10392490) ; 中佐 智幸 広島大学, 病院(医), 講師 (60467769)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 特発性大腿骨頭壊死症 / microRNA / 診断マーカー

Outline of Final Research Achievements

We evaluated disease specific microRNA in the synovial fluid of the patients with idiopathic osteonecrosis of the femoral head (ONFH) for detection of the new biomarker. We detected several microRNAs including let7c, miR-30, miR-31, miR-150, miR-210 which were highly up-regulated in ONFH patients compared with control. Furthermore, our findings revealed that the expression level of up-regulated miRNAs didn't affect the ONFH stage. We tried to detect the association specific miRNA such as steroid-related, alcohol related, but there were no specific miRNAs with statistical significance, which might be caused from small number of the ONFH patients, especially the ONFH patients without steroid and alcohol relation.

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

特発性大腿骨頭壊死症症例の関節液中には, 疾患特異的なmicroRNA(let7c, miR-30, miR-31, miR-150, miR-210)が高発現しており、さらに壊死症のどのstageにおいても恒常的に高発現していることが示された。これらの疾患特異的と考えられるmicroRNA(単独もしくは複数のmicroRNAを組み合わせることで)を用いることで、これまで少なからず誤診されてきた、特発性大腿骨頭壊死症に対し新たな診断マーカーになる可能性があることが示された。