2022 Fiscal Year Final Research Report

Analysis of extracellular vesicles using patient derived xenograft

Research Project

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Project/Area Number	19K09838
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 56040:Obstetrics and gynecology-related
Research Institution	Osaka Medical and Pharmaceutical University
Principal Investigator	Tomohito Tanaka 大阪医科薬科大学, 医学部, 講師 (90411363)
Co-Investigator(Kenkyū-buntansha)	林正美大阪医科薬科大学, 医学部, 准教授 (00551748) 大道正英大阪医科薬科大学, 医学部, 教授 (10283764) 田中良道大阪医科薬科大学, 医学部, 講師 (10625502) 恒遠啓示大阪医科薬科大学, 医学部, 講師 (70388255) 佐々木浩大阪医科薬科大学, 医学部, 講師 (80432491) 古形祐平大阪医科薬科大学, 医学部, 助教 (80829953) 寺井義人大阪医科薬科大学, 医学部, 非常勤講師 (90278531)
Project Period (FY)	2019-04-01 – 2023-03-31
Keywords	細胞外小胞 / PDX / マイクロRNA
Outline of Final Research Achievements	This study aimed to identify cancer-specific miRNAs as novel ovarian clear cell carcinoma (OCC) biomarkers using tissue-exudative extracellular vesicles (Te-EVs). Te-EVs were collected from four patients with OCC on one side and a normal ovary on the other side. Microarray analysis was performed to identify cancer-specific miRNAs in Te-EVs. Serum samples obtained before and after surgery from patients with OCC and atypical endometrial hyperplasia (AEH) (controls) were compared. Thirty-seven miRNAs were > 2-fold upregulated on the OCC side compared with the normal ovarian side. The levels of six EV miRNAs were significantly decreased in postoperative OCC serum compared to those in preoperative OCC serum. In contrast, no significant change was observed between the pre and post-operative values in the control group. We identified OCC tissue-specific miRNAs in the EVs secreted by OCC tissues. These EV miRNAs have potential for use as biomarkers for the early diagnosis.
Free Research Field	Molecular oncology
Academic Significance and Societal Importance of the Research Achievements	本研究では癌が放出する細胞外小胞内の癌特異的マイクロRNAを同定した。さらに、細胞外小胞自体が転移や浸潤に影響を与えていることを確認した。これらの癌組織から放出された細胞外小胞をターゲットにして、癌の早期発見や、新薬の開発の可能性が示唆された。