2021 Fiscal Year Final Research Report
Gene expression analysis of innate immunity mechanisms in the cochlea with acute sensorineural hearing loss
| Project/Area Number |
19K09845
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
假谷 伸 岡山大学, 医歯薬学域, 准教授 (10274226)
菅谷 明子 岡山大学, 大学病院, 助教 (20600224)
高原 潤子 岡山大学, 医学部, 技術専門職員 (80448224)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 蝸牛 / 急性音響性障害 / 遺伝子発現解析 / 免疫機能 / 炎症機能 / Fkbp5 / インターフェロン / インフラマソーム |
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| Outline of Final Research Achievements |
Molecular mechanisms of inflammation and innate immunity were clarified by gene expression analyses in the mouse cochlea following intense noise exposure. An immunity-related gene, Fkbp5 was upregulated in the cochlea at 3 hours post-noise trauma compared to the cochlea without noise exposure. Ten interferon-inducible genes were upregulated in the cochlea at 12 hours post-noise trauma. Expressions of 84 genes related to inflammasome were analyzed by realtime RT-PCR array, but only 4 genes of these were significantly upregulated in the cochlea at 12 hours post-noise trauma. In our dataset of RNA-seq and DNA microarray, expressions of 2285 long non-coding RNAs were detected in the cochlear tissues. Four long non-coding RNAs, 8030423F21Rik, Gm5083, A630001G21Rik, and Gm19299 were upregulated or downregulated in the cochlea following acoustic trauma. The relationship between these long non-coding RNAs and inflammation/immunity has not been reported in previous papers.
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| Free Research Field |
医歯薬学
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| Academic Significance and Societal Importance of the Research Achievements |
急性感音難聴は比較的頻繁にみられ、かつ難治性の病態である。一般にその治療にはステロイド投与が行われ、ステロイドの作用機序としては炎症、免疫反応を制御すると想定されている。当研究では急性感音難聴（急性音響性障害）を発症したマウスの内耳で、どの様な炎症、免疫機能がかかわっているか、遺伝子発現のレベルで検討した。免疫関連遺伝子Fkbp5の発現と難聴発症の関係を検討し、どの様な機能的カテゴリーの炎症・免疫関連遺伝子群が難聴発症に関わるか検討した。当研究の成果は急性感音難聴の病態解明と治療法開発のために役立つ。
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