2022 Fiscal Year Final Research Report
Analysis of mucosal immune responses in airway system by aging
Project/Area Number |
19K09872
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 56050:Otorhinolaryngology-related
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Research Institution | Oita University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
安倍 伸幸 大分大学, 医学部, 客員研究員 (10433054)
鈴木 正志 大分大学, 医学部, 教授 (60211314)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | インフルエンザ菌 / 粘膜免疫 / 加齢 / 上気道粘膜 / T細胞分化 |
Outline of Final Research Achievements |
In this study, we analyzed changes of mucosal immune responses in upper airway by aging against outer membrane protein (OMP) from nontypeable Haemophilus influenzae, focusing on the differentiation of T cells. Mice (5 weeks, 6 months, 1 year old mice) were vaccinated with OMP and cholera toxin three times seven days apart. Mononuclear cells were collected from middle ear, nasal mucosa, nasal associated lymphoid tissue, cervical lymph node, and spleen to analyze T cell differentiation by flow cytometry at day 7 after the immunization. Non-immunized mice at each age were served as a control. In CD4-positive T cells, the memory T cell ratio was increased in upper airway mucosa in young mice after the immunization, and also, the effector T cell ratio was increased markedly in lymphoid tissues in young mice after the immunization. It was suggested that the T cell immune responses against OMP in the upper airway mucosa and lymphoid tissues may decrease by aging.
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Free Research Field |
上気道粘膜免疫
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Academic Significance and Societal Importance of the Research Achievements |
小児のみならず高齢者を含めて、無莢膜型インフルエンザ菌は上気道のみならず下気道においても感染症を発症する起炎菌であり、鼻咽腔におけるインフルエンザ菌の定着が発症の引き金になりうることが推測されている。このような背景からインフルエンザ菌ワクチンの開発は、小児のみならず高齢者においても急務であり重要な意義を持つものと思われる。加齢に伴って局所から全身免疫系を含めたT細胞における免疫応答機能が低下し、感染抵抗性が低下することはよく知られている。今回、加齢に伴う上気道粘膜免疫応答をT細胞含めた免疫応答について解析することにより、インフルエンザ菌粘膜ワクチン開発への応用につなげることが可能となる。
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