2022 Fiscal Year Final Research Report
The objective evaluation of vestibular migraine
| Project/Area Number |
19K09877
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永田 栄一郎 東海大学, 医学部, 教授 (00255457)
室伏 利久 帝京大学, 医学部, 教授 (30242176)
北原 糺 奈良県立医科大学, 医学部, 教授 (30343255)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 前庭 |
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| Outline of Final Research Achievements |
The pain stimulation can probably induce excitatory cell death in the vestibular cells depending on the time and concentration. TLR7 expression in Si-STRPA1 cells was reduced related to the concentration of TRPA1. These results indicate the possible crosslink between TRP1 and TKW7. In addition to thatm, miRA\NA let-7b regulated the expression of TRPA1 via TLR7. The expression of P62 gradually increased up to 24 hours after the stimulation. TRL7 agonist loxoribine increased the expression of not only TLR7 but also TRPA1. The cell excitatory marker p-EKP also increased in the same condition. These results indicate that there is the crosslink between TLR7 and TRPA1. TLR7 is functioning as the driver gene of TRPA1 via this crosslink.
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| Free Research Field |
耳鼻咽喉科
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| Academic Significance and Societal Importance of the Research Achievements |
前庭上皮細胞特異的に痛み刺激により過興奮性細胞死を誘導するexosome(exo)-miRNAを探索するため、エクソソーム表面マーカーCD81の発現を確認し、また電子顕微鏡下においてエクソソームが正確に単離されたことを確認した。これらより、 AITC処理細胞由来エクソソームが前庭上皮細胞自体の過興奮性細胞死連鎖を誘導する可能性が示唆している。以上の結果より前庭上皮細胞の障害により分泌されたエクソソームは、中枢の神経-血管-グリアユニットにボトムアップシグナルを送る可能性が示唆された。
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