2022 Fiscal Year Final Research Report
Exploration of Hearing Loss Prevention and Treatment Strategies using Cultured Cochlear Lateral Wall and Live Cell Fluorescence Imaging
| Project/Area Number |
19K09898
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 難聴 / ストレス / 中間細胞 / 蝸牛 / 外側壁 / メラノサイト / 抗酸化剤 / 線維芽細胞成長因子 |
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| Outline of Final Research Achievements |
The cells in the cochlear lateral wall cell of the inner ear play an essential role in maintaining normal hearing. In this study, we focused on the intermediate cells within the lateral wall cell population and initiated our investigation. Observing the temporal morphological changes of intermediate cells in cultured tissues suggested that these cells may have diverse functions beyond substance transport within the cochlea. It was also suggested that melanocyte growth factors and commercially available antioxidants marketed as supplements potentially have a mitigating effect on the decreased survival rate of intermediate cells due to oxidative stress or aging. Furthermore, fibroblast growth factor 2 (FGF2), currently used clinically as a periodontal tissue regenerative agent, demonstrated cell proliferative effects on the outer wall tissues, suggesting its potential application in otology.
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| Free Research Field |
耳科学
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| Academic Significance and Societal Importance of the Research Achievements |
聴覚にかかわる神経回路の最も末梢にある蝸牛有毛細胞を再生させる研究は世界中で非常に熱心に行われている。しかし、著明な効果を有する薬剤が臨床現場で汎用されるには長い時間と莫大な費用を要することが予想される状況である。一方、外側壁細胞群は薬剤により再生や増殖を促すことが比較的容易であることが示された。外側壁細胞群が正常に機能しなければ有毛細胞も正常な機能、生存は不可能である。急性難聴や加齢性難聴など幅広い病態を含む難聴の新規予防法、治療法を早期に臨床現場へ導入するためには蝸牛外側壁細胞群をターゲットにした研究が有用であることが示された。
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