Development of a radical regenerative therapy for radiation-damaged tissue using mesenchymal stem cells and vascular endothelial progenitor cells

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Jichi Medical University
• Principal Investigator: Kamochi Hideaki 自治医科大学, 医学部, 客員研究員 (10586366)
• Co-Investigator(Kenkyū-buntansha): 吉村 浩太郎 自治医科大学, 医学部, 教授 (60210762)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 再生医療 / 放射線障害 / 間葉系幹細胞 / 血管内皮前駆細胞 / 創傷治癒

Outline of Final Research Achievements

A chronic radiation skin disorder model was created by irradiating the back of a 7-week-old male nude mouse with 5 Gy twice a week for a total of 40 Gy and excising the skin 3 months after the end of irradiation. ASC and AEPC were administered alone or in combination to evaluate the wound healing ability.
The combined administration of ASC and AEPC showed a marked improvement in wound healing ability, but engraftment of administered cells could not be confirmed in any of the groups. It was suggested that the improvement of wound healing ability is greatly affected by the humoral factors secreted by the cells even if the cells themselves do not engraft.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

脂肪組織から培養した脂肪幹細胞と血管内皮前駆細胞を局所投与することにより放射線障害組織の創傷治癒能を改善することができる可能性が示された。また今回の研究結果からは、細胞自体を投与しなくとも細胞が放出する物質のみを投与すれば同等に創傷治癒能を改善させることができる可能性が示唆された。細胞自体ではなく細胞が放出する物質であれば工業的に大量生産することも可能であり、適応がさらに広がる可能性がある。