2021 Fiscal Year Final Research Report

Ethanol pretreatment of nanoPGA/PCL composite scaffolds enhances human chondrocyte development in the tissue-engineered auricle constructs

Research Project

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Project/Area Number	19K10020
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 56070:Plastic and reconstructive surgery-related
Research Institution	Kindai University
Principal Investigator	Noritaka Isogai 近畿大学, 医学部, 教授 (90203067)
Co-Investigator(Kenkyū-buntansha)	諸富公昭近畿大学, 医学部, 准教授 (10388580) 楠原廣久近畿大学, 医学部, 講師 (50388550) 和田仁孝和歌山県立医科大学, 医学部, 准教授 (10460883)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	Ethanol treatment / scaffold / cartilage / tissue engineering / ear / hydrophilicity
Outline of Final Research Achievements	Human chondrocytes were seeded onto three-dimensional bioresorbable composite scaffolds (PCL with surface coverage of nanoPGA) either with or without ethanol pretreatment and then implanted into athymic mice for 10 and 20 weeks. After implantation for 10 weeks, constructs of human auricular chondrocytes seeded onto ethanol-treated scaffolds were covered with glossy cartilage. RT-qPCR analyses of chondrocytes grown on ethanol-treated scaffolds showed greater expression levels for several cartilage-related genes compared to cells developed on untreated scaffolds with statistically significantly increased SRY-box transcription factor 5 (SOX5) and decreased interleukin-1α (inflammation-related) expression levels. Findings suggest enhancing nanoPGA/PCL scaffold hydrophilicity utilizing ethanol improves the cellular microenvironment and cartilage regeneration in tissue-engineered auricle constructs.
Free Research Field	形成外科学
Academic Significance and Societal Importance of the Research Achievements	耳介軟骨の再生誘導技術を臨床応用する上で、耳介特有の３次元形状が単に再現されるのみでなく、長期的形状が維持され、成長や経年変化についても考慮されていることが望まれる。今回の研究より、エタノール表面改質した複合型吸収性スキャフォールド（耳介足場）は、高い軟骨再生能と三次元耳介形状を長期維持するために必要な高い力学的特性を有しており、今後、本表面改質技術は３次元形状軟骨の再生誘導を臨床応用する上で、有用な基盤技術になると考えられる。