Functional enhancement of MSCs based on analysis of expression profiles of secreted factors

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Hitomi Takada 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (80641068)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 間葉系幹細胞 / シングルセル解析 / 分泌性因子 / 下肢虚血

Outline of Final Research Achievements

Although mesenchymal stem cells holds great promise in regenerative medicine to treat various inflammatory diseases or ischemic diseases, there are no standards for cell isolation procedure and culture method. Here we analyzed the gene expression profile of freshly isolated stromal vascular fraction (SVF), SVF cultured under the adherent condition, and SVF cultured under the floating condition by single-cell RNA-seq. In addition, we injected those cells into limb ischemia model mice and analyzed their therapeutic efficacy. We found that SVF cultured under the floating condition maintained the gene expression profiles of freshly isolated SVF, and that SVF cultured in different culture conditions exhibited different expression profiles of secreted factors.

Free Research Field

幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

間葉系幹細胞を用いた再生医療は、様々な炎症性疾患や虚血性疾患における新たな治療法として急速に研究が進められている。ところが、間葉系幹細胞の培養方法や選別方法は統一されておらず、どのような性質を持った間葉系幹細胞が高い治療効果を持つのかについて包括的な研究はほとんどなされていない。本研究は、培養条件の異なる間葉系幹細胞の遺伝子発現をシングルセルレベルで比較し、下肢虚血モデルマウスを用いてそれらの治療効果比較した。