2019 Fiscal Year Research-status Report
Investigation of chromatic remodeling in osteoclast function for therapeutic targeting of bone remodeling balance
| Project/Area Number |
19K10044
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| Research Institution | Ehime University |
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Principal Investigator |
李 智媛 愛媛大学, プロテオサイエンスセンター, 助教(特定教員) (70711274)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | SWI/SNF / BAF155 / Histomorphometry / Rank / Lysosome |
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| Outline of Annual Research Achievements |
Generation of cKO mice in FY2019; SWI/SNF (chromatin remodeler) complex is essential gene and conventional deletion leads to embryonic lethality. As planed in proposal, we generated LysM-Cre;Baf155f/f and Rank-Are; Baf155f/f mice which are specific deletion for osteoclast progenitor and precursor, respectively. We studied bone remodeling in adult mice of 8weeks female and male. We examined the bone mineral density by microCT and bone histomorphometry using femoral bone sections. Each parameters for osteoclastic and bone resorption were intensively score.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Our analysis is progressing as planed in proposal. Currently we design for RNA-Seq analysis and will perform the data mining.
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| Strategy for Future Research Activity |
From analysis of RNA-seq (or ChIP-seq if we need), we will analysis for data mining.
We also examine the osteoclast ontogeny. For in vitro assays, we harvest bone marrow cells from mouse tibia, and then culture them with M-CSF and RANKL and allow to proliferate and differentiation into osteoclast activation. We will perform cell morphology observation during cell activation, which are affect from chromatin remodeling.
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| Causes of Carryover |
Transcriptomic analysis like RNA-Seq and ChIP-Seq will perform in FY2020.
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