2020 Fiscal Year Research-status Report
Investigation of chromatic remodeling in osteoclast function for therapeutic targeting of bone remodeling balance
| Project/Area Number |
19K10044
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| Research Institution | Hokkaido University |
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Principal Investigator |
李 智媛 北海道大学, 歯学研究院, 助教 (70711274)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | SWI/SNF / BAF155 / Smarcc1 / Osteoclast / Bone / Chromatin remodeler |
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| Outline of Annual Research Achievements |
This research we investigate the functional role of epigenetic chromatic remodelers, BAF155 in osteoclasts and bone metabolism. In FY2020, we have finished the RNA-sequencing analysis from LysM-Cre;Baf155 flexed mice, which is specific deletion of Baf155 expression in osteoclast progenitor. In particular, we performed RNA-seq. into two by two group: bone marrow macrophage and osteoclast precursor group from control and Cre-Specific deletion, subdividing the osteoclast ontogeny stages affected by chromatin remodeling. A gene ontology analysis demonstrated that the greatest number genes that were upregulated by Cre-deletion mouse in osteoclastogenesis were found in pathways related to lysosome, focal adhesion and osteoclast differentiation. We also identified the candidate genes from comparison analysis. We are validating the expression of osteoclast ontogeny in cultured cell.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
I have moved to new position at Hokkaido University from 2019 April. During this pandemic situation, all the animal facility has limitation to establish and expand the mouse line. The University now has just allowed to expand the mouse line, I can start to do mouse experiment this April. Slightly delayed than the scheduling, but the process is going on the way.
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| Strategy for Future Research Activity |
In FY2021, we apply ChIP-Sequencing to find potent transcription factors that interact with BAF complex and function under the control of osteoclast differentiation. ChIP-se combines chromatin immunoprecipitation with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. And we also apply the targeted proteomics approach to find protein from preselected group of proteins using mass spectrometer.
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| Causes of Carryover |
仕事の転勤とコロナパンデミックの影響で動物実験などの制限があり、予定より実験などの計画に遅延が生じた。FY2021にChIP seq.データー解析、たんぱく質同定、細胞培養、分子生物分析に使う予定。
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