2021 Fiscal Year Research-status Report
Investigation of chromatic remodeling in osteoclast function for therapeutic targeting of bone remodeling balance
| Project/Area Number |
19K10044
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| Research Institution | Hokkaido University |
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Principal Investigator |
李 智媛 北海道大学, 歯学研究院, 助教 (70711274)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | Osteoclast / BAF155 / Bone |
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| Outline of Annual Research Achievements |
In this research, we investigated the functional role of epigenetic chromatin remodeler, BAF155 in osteoclasts and bone metabolism. By analysis of microCT and bone histomorphometry of 16-week-old male mice, we confirmed that the Baf155 deficient mice significantly increased the TRAP5b and CTX which are the representative serum markers of bone resorption, and thereby decreased the bone mineral density. By using human osteoclasts, we demonstrated that BAF155 expression during osteoclast differentiation stages. The BAF155 expression was significantly decreased in mature osteoclast, not bone marrow macrophage or osteoclast precursor stage, in contrast BAF170 expression was increased. Also, we found several target candidates from RNA sequencing analysis. We are investigating the validation of candidates by RT-PCR and immunoblotting.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The research is proceeding as initially planned. Animal testing was slightly delayed because of Corona pandemic, but it is now proceeding according to the original plan by applying other experimental assay.
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| Strategy for Future Research Activity |
In further research period, we will apply and verify the osteoclast differentiation using candidates found by RNA sequencing analysis. Also, we will apply super-resolution microscopy to observe sub cellular organelle dynamics and cell morphology.
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| Causes of Carryover |
コロナ感染拡大防止の状況で、遺伝子組み替え実験動物の制作・輸送など制限され、実験実施・予算実行が予定より遅延されたため。
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