Investigation of chromatic remodeling in osteoclast function for therapeutic targeting of bone remodeling balance

2022 Fiscal Year Annual Research Report

• Project/Area Number: 19K10044
• Research Institution: Hokkaido University
• Principal Investigator: 李 智媛 北海道大学, 歯学研究院, 助教 (70711274)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: Osteoclast / Baf155 / Chromatin remodeler / sexual dimorphism

Outline of Annual Research Achievements

This study investigated the functional role of Baf155, an epigenetic chromatin remodeler in osteoclasts. In this fiscal year, we found that the effect of Baf155 cKO mice on bone mineral density (BMD) differed between sexes, even at the same age. Interestingly, microCT analysis of 8-week-old mice showed an increase in BMD only in male mice, but there was no difference in BMD at 16 weeks of age. Conversely, in 8-week-old female mice, there was no difference in BMD compared to the control group, but at 16 weeks of age, BMD parameter significantly increased in Baf155 cKO. Our results suggest that the epigenetic regulation of the chromatin regulator Baf155 on osteoclasts and bone differs according to sexual dimorphism.

Research Products

• [Journal Article] Association between an increased serum CCL5 level and pathophysiology of degenerative joint disease in the temporomandibular joint in females (2023)
  • Author(s): H Watanabe, T Iori, Ji-Won Lee, T Kajii, A Takakura, T Takao-Kawabata, Y Kitagawa, Y Maruoka, T Iimura
  • Journal Title: International journal of molecular sciences Volume: 24 Pages: －
  • DOI: 10.3390/ijms24032775
• [Journal Article] Evolution of multiple comics approaches to define pathophysiology of pediatric acute respiratory distress syndrome (2022)
  • Author(s): Jane E Whitney, In-Hee Lee, Ji-Won Lee, Sek Won Kong
  • Journal Title: Elife Volume: 11 Pages: －
  • DOI: 10.7554/eLife.77405
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Lipid-Binding Defective Dynamin 2 mutant in Charcot-Marie-Tooth Disease impairs proper actin bundling and actin organization in Glomerular podocytes (2022)
  • Author(s): 2.Hamasaki E, Wakita N, Yasuoka H, Nagaoka H, Morita M, Takashima E, Uchihashi T, Takeda T, Abe T, Lee JW, Iimura T, Saleem MA, Ogo N, Asai A, Narita A, Takei K, Yamada H
  • Journal Title: Frontiers in cell and development biology Volume: 10 Pages: －
  • DOI: 10.3389/fcell.2022.884509
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Histone H3K27 demethylase, Utx, regulates osteoblast-to-osteocyte differentiation (2022)
  • Author(s): Yuhan Xia, Aoi Ikedo, Ji-Won Lee, Tadahiro Iimura, Kazuki Inoue, Yuuki Imai
  • Journal Title: Biochemical and biophysical research communications Volume: 590 Pages: 132-138
  • DOI: 10.1016/j.bbrc.2021.12.102
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expansion of the osteocytic lacunar-canalicular system involved in pharmacological action of PTH revealed by AI-driven fluorescence morphometry in female rabbits (2022)
  • Author(s): A Takakura, T Sato, Ji-Won Lee, K Hirano, R Takao-Kawabata, T Ishizuka, T Iimura
  • Journal Title: Scientific reports Volume: 12 Pages: －
  • DOI: 10.1038/s41598-022-20793-5