• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2021 Fiscal Year Annual Research Report

Gel vaccine designed to co-stimulate both humoral and cellular immunity ideal for elderly vaccination

Research Project

Project/Area Number 19K10097
Research InstitutionNihon University

Principal Investigator

Cueno Marni  日本大学, 歯学部, 専修研究員 (20569967)

Project Period (FY) 2019-04-01 – 2022-03-31
Keywordsgingival vaccine / influenza A H3N2 / influenza B/Yamagata
Outline of Annual Research Achievements

We substituted the pneumolysin protein antigen with the Wuhan original SARS CoV 2 spike protein antigen since the pneumolysin protein was no longer commercially available while the original SARS CoV 2 spike protein is readily available. In this regard, we were able to successfully design molecular structures of our target antigens, namely: hemagglutinin protein of influenza A H3N2 strain, spike protein of the original SARS CoV 2, and hemagglutinin protein of influenza B/Yamagata strain. Subsequently, we were able to design and predict the ideal antigen:gel ratio (100 microgram antigen: 0.1 milligram xanthum gel) for all of our target proteins. Based on computer-based prediction, gel encapsulation of all protein antigens will make them room temperature stable. Additionally, optimization of our gingival vaccination strategy was likewise successful. Application of the antigen:gel mix along the molar teeth of the experimental rat was found to be ideal to induce an antibody response. Optimization of the standard CNS demyelination network design relative to our study is still on-going.

  • Research Products

    (2 results)

All 2021

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Structural Insights on the SARS-CoV-2 Variants of Concern Spike Glycoprotein: A Computational Study With Possible Clinical Implications2021

    • Author(s)
      Marni E. Cueno, Kenichi Imai
    • Journal Title

      Frontiers in Genetics

      Volume: 12 Pages: 773726

    • DOI

      10.3389/fgene.2021.773726

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Structural differentiation of the spike glycoprotein form SARS-CoV-2 COVID-19 variants of concern compared to other human-infecting coronaviruses: A computational study with clinical implications.2021

    • Author(s)
      Marni E. Cueno, Kenichi Imai
    • Organizer
      25th Congress of the Asia Pacific Society of Respirology
    • Int'l Joint Research

URL: 

Published: 2022-12-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi