2021 Fiscal Year Final Research Report

Development of new pathological diagnosis method of odontogenic cyst using the findings of histogenesis and epithelial-mesenchymal histopathology

Research Project

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Project/Area Number	19K10100
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57020:Oral pathobiological science-related
Research Institution	Aichi Gakuin University
Principal Investigator	Maeda Hatsuhiko 愛知学院大学, 歯学部, 教授 (30175591)
Co-Investigator(Kenkyū-buntansha)	吉田和加愛知学院大学, 歯学部, 講師 (10513210) 杉田好彦愛知学院大学, 歯学部, 准教授 (20367618) 久保勝俊愛知学院大学, 歯学部, 准教授 (60329604)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	歯原性嚢胞 / 病理診断 / 上皮間葉転換 / 口腔組織発生 / 組織アレイ / サイトケラチン / 免疫染色
Outline of Final Research Achievements	Histopathological findings of odontogenic cysts are similar, and routine pathological diagnosis is often difficult because they are often accompanied by secondary inflammation. This study aimed to develop a simple, reliable, and practical immunostaining-based pathological diagnosis method for odontogenic cysts by applying embryological findings and epithelial-mesenchymal histopathology. As a result, we found that a combination of immunostaining for CK-13, CK-17, Ki-67, EGFR, Gli-2, and Loricrin is the optimal combination of immunostaining that can distinguish odontogenic keratocyst from other odontogenic cysts in pathological diagnosis. This pathological diagnosis method will greatly contribute to the treatment of odontogenic cysts.
Free Research Field	口腔病理学
Academic Significance and Societal Importance of the Research Achievements	顎骨内嚢胞の治療において、歯原性角化嚢胞は再発率が高いことが問題であり、歯原性角化嚢胞との鑑別を正確におこなうことが重要である。しかし、歯原性嚢胞の裏装上皮は病理組織学所見が類似しており、二次的な炎症を伴った場合の正確な病理診断は極めて難しい。本研究では、歯原性嚢胞の簡便で信頼性の高い実践的な病理診断方法を開発することを目指している。この病理診断方法により、日常の歯原性嚢胞の診断・治療に大きく寄与し、ひいては国民の健康維持および医療費の負担軽減につながる。