2021 Fiscal Year Final Research Report
Functions of Mkx in periodontal ligament homeostasis
Project/Area Number |
19K10145
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 57030:Conservative dentistry-related
|
Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
HARADA HIROYUKI 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (40343149)
|
Co-Investigator(Kenkyū-buntansha) |
青木 章 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (30302889)
淺原 弘嗣 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70294460)
|
Project Period (FY) |
2019-04-01 – 2022-03-31
|
Keywords | Mkx / 歯根膜 |
Outline of Final Research Achievements |
The present study aimed to clarify the effects of a Mkx deficiency on PDL cellular heterogeneity and differences between gene expression in PDL tissues from wild-type (WT) and Mkx knockout rats using single-cell RNA sequencing. We identified 12 cell clusters comprising mesenchymal cells and macrophages. The expression of Mkx and scleraxis, was mutually exclusive, and partitioned mesenchymal cell clusters into Mkx and Scx types that dominantly expressed proteoglycans and elastic fibers, and type 1 and 3 collagen, respectively. Ossificationrelated genes were upregulated in mesenchymal cell and osteoblast clusters with more Mkx-/- than Mkx+/+ PDLs. Increased number of cells and inflammatory mediators were observed in macrophage clusters of Mkx-/- PDL. These results suggested that Mkx plays an important role in maintaining PDL homeostasis by regulating specific cell populations and gene expression.
|
Free Research Field |
口腔外科学
|
Academic Significance and Societal Importance of the Research Achievements |
以上の成果はMkxが歯周炎や骨性癒着に関与していることを示唆しており、Mkxの発現制御が歯周治療における新たな治療になりうることが示唆された。
|