Elucidation of the molecular mechanism of Herpes Simplex Virus -1infection using Raman spectroscopy

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K10170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Adachi Keiji 京都府立医科大学, 医学(系)研究科(研究院), 助教 (70457951)
• Co-Investigator(Kenkyū-buntansha): 足立 哲也 京都府立医科大学, 医学(系)研究科(研究院), 講師 (10613573) ; 山本 俊郎 京都府立医科大学, 医学(系)研究科(研究院), 講師 (40347472) ; 金村 成智 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70204542) ; PEZZOTTI G. 京都工芸繊維大学, 材料化学系, 教授 (70262962) ; 扇谷 えり子 京都府立医科大学, 医学(系)研究科(研究院), 特任准教授 (80300820)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: ラマン分光法 / 単純ヘルペスウイルス1型 / ラマンイメージング / HSV-1 / ミエリン / 神経伝達物質

Outline of Final Research Achievements

In this study, we used Raman spectroscopy to noninvasively visualize the dynamic changes in host cells and virus particles associated with herpes simplex virus type 1 (HSV-1) infection. Elucidate pathology. The infection mechanism of HSV-1 that causes herpes labialis is largely unknown, and it is necessary to monitor virus particle dynamics in host cells to understand in detail the changes associated with virus entry and reactivation. However, most of the conventional research methods destroy cells, and it has been difficult to monitor the dynamic changes that occur between the host and the virus as they are. We focused on Raman spectroscopy, which is non-staining, minimally invasive, and capable of molecular-level analysis. If Raman spectroscopy can be used to simultaneously analyze changes at the molecular level of viruses and neurons that occur during primary infection and reactivation in real time, it will be possible to identify molecules related to infection and reactivation.

Free Research Field

口腔外科学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、HSV-1感染症の病態解明だけでなくアシクロビルに代わる新規抗ヘルペス薬やワクチンの開発に繋がることが期待できる。