New strategy for tissue regeneration of salivary gland by activation of the hypoxic response

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Tokyo Dental College
• Principal Investigator: Kasahara Masataka 東京歯科大学, 歯学部, 教授 (30328265)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 再生医療 / ドラッグデリバリーシステム / 低酸素応答

Outline of Final Research Achievements

Atelocollagen-gelatin (ACG) sponge was fabricated from atelocollagen and gelatin by lyophilization without introducing toxic substances. This study aimed to investigate the effects of heat treatment on the 3-dimensional structural stability of ACG sponge biomaterial. ACG sponge samples were fabricated and heat treated at 125oC for 12 h in the vacuum. The results revealed that heat treatment did not affect porosity, pore size and mechanical compressive strength. Heat-treated ACG sponge showed decreased absorbance and peak shift of amid I (C=O) stretches, slightly higher water uptake degree and significantly decreased in vitro
degradation rate. Moreover, heat-treated ACG sponge maintained good 3-dimensional surface morphology and porous microstructure throughout 7 days, while non-heat-treated ACG sponge collapsed in less than 24 h. The human mesenchymal stromal cells (hMSCs) were shown to adhere and grow well on heat-treated ACG sponges.

Free Research Field

薬理学

Academic Significance and Societal Importance of the Research Achievements

全身投与の薬物は、全身に作用を及ぼし、時に有害な作用を引き起こす可能性がある。一方、治療したい局所に薬物や幹細胞を適用する方法は、治療効果を高めるとともに、生体に及ぼす有害作用を軽減することができる。今回は治療効果を高めるために、足場としてのatelocollagen/gelatin spongeを、熱架橋により薬物担持能力の向上、形態維持能力の向上が認められた。このことは治療の効果を高めることにつながる。