Bone and tooth differentiation induced by BMP and rapamycin

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K10177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Tsurumi University
• Principal Investigator: Oida Shinichiro 鶴見大学, 歯学部, 名誉教授 (10114745)
• Co-Investigator(Kenkyū-buntansha): 寺島 達夫 鶴見大学, 歯学部, 非常勤講師 (20114770) ; 山本 竜司 鶴見大学, 歯学部, 講師 (20410053) ; 唐木田 丈夫 鶴見大学, 歯学部, 准教授 (40367305) ; 大熊 理紗子 鶴見大学, 歯学部, 学部助手 (50804887) ; 斉藤 まり 鶴見大学, 歯学部, 助教 (60739332)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: ラパマイシン / BMP / 骨の再生 / 歯の再生 / 石灰化

Outline of Final Research Achievements

We investigated the interaction between rapamycin and bone morphogenetic protein (BMP) in the formation of teeth and bone. In three-dimensional cultures using a cell line resembling pig dental pulp (PPU-7), administration of BMP resulted in the observation of calcified tissue surrounded by spherical cell layers, with further enhancement of calcification upon rapamycin administration. Similar results were confirmed in experiments using mouse tooth germ organ culture systems. In experiments using mouse osteoblast cell line cells (C2C12), a significant synergistic effect was observed with the administration of both substances, leading to the promotion of bone formation. The differentiation induction pathway of osteoblasts was examined through experiments using various inhibitors. These findings suggest the potential application of rapamycin and BMP in regenerative therapies for bone and teeth.

Free Research Field

分子生化学

Academic Significance and Societal Importance of the Research Achievements

歯や骨の分化に対するBMPとラパマイシンの作用を検討し、学術的にはBMPは歯と骨の細胞増殖と分化を促進し、ラパマイシンは細胞増殖を抑制するが、BMPとの相乗効果で著しく分化を促進するという結果が得られた。これらの結果から社会的意義としては、ラパマイシンは歯や骨の形成過程で、BMPの分化誘導効果を促進し、再生治療に活用できる可能性が示唆された。