Research of properties and functions of exosomes derived from human dental pulp cells and pulp-derived pluripotent stem cells.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K10263
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Gifu University
• Principal Investigator: HATAKEYAMA Daijiro 岐阜大学, 医学部附属病院, 講師 (60377653)
• Co-Investigator(Kenkyū-buntansha): 川口 知子 (武田知子) 岐阜大学, 医学部附属病院, 医員 (30509815) ; 飯田 一規 岐阜大学, 大学院医学系研究科, 講師 (30585237) ; 柴田 敏之 岐阜大学, 大学院医学系研究科, 教授 (50226172)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 歯髄細胞 / エクソソーム

Outline of Final Research Achievements

We induced induced pluripotent stem cells (iPS cells) from about 300 human dental pulp cells (DPCs) that we have established and possess. To ensure that the exosomes of induced iPS cells do not contain serum-derived components or feeder cell-derived components, DPCs were prepared under serum-free culture conditions, and iPS cells were prepared from these cells under serum-free culture conditions without feeder cells. The safety and effects of exosomes on regenerative therapy, especially on bone regeneration, were investigated for future applications.

Free Research Field

外科系歯学

Academic Significance and Societal Importance of the Research Achievements

髄細胞のエクソソームを用いて骨の再生や治癒を検討した研究はまだ報告がなく独自性があると考える。そのために、我々が樹立したヒト歯髄細胞株が約300ラインをすでに保管されていること、また、無血清条件下での細胞培養法と無血清・フィーダー細胞を用いないiPS細胞誘導・培養法を我々が確立していることは、本研究を進めるうえでその意義は大きく、再生治療への応用に向けて歯髄細胞の医療資源としての価値は高いと考える。