Circadian rhythm determination of oral inflammation using fluorescence imaging

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K10394
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: NISHIDE Shinya 北海道医療大学, リハビリテーション科学部, 講師 (40451398)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 概日リズム / 蛍光イメージング / 炎症

Outline of Final Research Achievements

For the future purpose of measuring the circadian rhythm observed in the inflammatory response of the dental pulp, in this study, the inflammatory marker MAPK activity was measured by FRET using mammalian cultured cell lines. In this study, we selected ERK and JNK from the MAPK molecule group, created biosensors, and transfected them into cultured cells. When cells were treated with proinflammatory substances at various times, MAPK responses were shown to change in a time-dependent manner. In addition, with the aim of introducing biosensors into animals and setting up a system to analyze the relationship between circadian rhythms and inflammatory responses in animals, we constructed a system to measure the behavioral rhythms of mice 24 hours a day, and measured the circadian rhythms.

Free Research Field

生理学

Academic Significance and Societal Importance of the Research Achievements

体温やホルモン分泌、唾液分泌など体の機能の多くには約24時間周期の概日リズムがある。夜型や不規則な生活習慣は概日リズムを乱し、肥満や生活習慣病の原因となることが過去の研究で明らかになっている。本研究は概日リズムの乱れが虫歯など口腔内の炎症性疾患の症状に影響するかどうかを検討するために行った。本研究において細胞内で炎症時にはたらく分子の挙動を蛍光タンパク質で観察することができるシステムを構築することができ、口腔内の炎症はもとより全身の様々な炎症性疾患の一日の中の変動を分析することが可能になった。