Establishment of the Chairside PCR Method and Development of RED Complex Sterilization Therapy in Preventive Dentistry

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K10472
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57080:Social dentistry-related
• Research Institution: Tsurumi University
• Principal Investigator: Yamada Hidenori 鶴見大学, 歯学部, 助教 (60240032)
• Co-Investigator(Kenkyū-buntansha): 村田 貴俊 鶴見大学, 歯学部, 講師 (10313529) ; 花田 信弘 鶴見大学, 歯学部, 教授 (70180916) ; 宮之原 真由 鶴見大学, 歯学部, 学部助手 (70460186) ; 野村 義明 鶴見大学, 歯学部, 学内教授 (90350587)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: REDコンプレックス / リアルタイムPCR / 唾液

Outline of Final Research Achievements

Colonization by several oral pathogens and the onset of oral diseases, such as dental caries and periodontal diseases, are closely related. Therefore, the analysis of pathogens in oral specimens would be helpful for the risk assessment of oral diseases. We developed a rapid multiplex real-time polymerase chain reaction (PCR) method using a portable device and newly designed probe/primer sets to detect the oral pathogens Streptococcus mutans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. The theoretical minimum detectable cell numbers of S. mutans, P. gingivalis, T. denticola, and T. forsythia were 1, 1, 4, and 3, respectively. The multiplex real-time PCR system simultaneously detected the colonization of S. mutans and P. gingivalis in human saliva.
These results suggest that the multiplex real-time PCR system may be useful for the risk assessment of oral diseases.

Free Research Field

口腔衛生学

Academic Significance and Societal Importance of the Research Achievements

歯周病原因菌で「Red Complex」であるP. gingivalis、Treponema denticola、および Tannerella forsythiaは、これまでキットによるリスク判定や、選択培地による嫌気性培養が必要で培養および判定に時間を要していた。今回の研究では、唾液からリアルタイムPCRデバイスにより、チェアサイドでこれらの感染とリスク管理を行うための特異的なブローブ、プライマーの設計を行い評価した。唾液を検体とした臨床における迅速なリスク評価に適している。