2022 Fiscal Year Final Research Report

Multicenter countywide surveillance of antimicrobial-resistant bacteria and development of a method to assess the risk of transmission through bacteriological analysis

Research Project

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Project/Area Number	19K10596
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 58020:Hygiene and public health-related: including laboratory approach
Research Institution	Tohoku University
Principal Investigator	Tokuda Koichi 東北大学, 大学病院, 准教授 (10518400)
Co-Investigator(Kenkyū-buntansha)	吉田眞紀子東北大学, 大学病院, 助教 (10543225)
Project Period (FY)	2019-04-01 – 2023-03-31
Keywords	薬剤耐性菌 / サーベイランス / 薬剤耐性遺伝子 / スクリーニング / 抗菌薬適正使用
Outline of Final Research Achievements	We conducted research on MRSA and CRE in Miyagi Prefecture, which had not previously been investigated in Miyagi Prefecture, and presented the epidemiological and bacteriological information we obtained at a national conference. The results of antimicrobial susceptibility analyses of approximately 3,000 CRE isolates showed that CRE resistant to ceftazidime and cefoperazone sodium sulbactam sodium could be carbapenemase-producing Enterobacteriaceae (CPE), which produce enzymes that inactivate carbapenems, when they are both resistant to ceftazidime and cefoperazone sodium sulbactam sodium. -This method was used to identify CREs that were resistant to both carbapenem sodium and sodium bicarbonate. The accuracy of this method for screening CRE for CPE was excellent, with a sensitivity of 100% and specificity of 46%, which verified the usefulness of this method.
Free Research Field	感染制御学
Academic Significance and Societal Importance of the Research Achievements	これまで宮城県で明らかにされていなかったMRSAおよびCREについて、分布などの疫学情報や毒素および耐性遺伝子などの細菌学的情報が明らかとなった。また、全国においても検出例が稀とされている耐性菌であるNDM-5メタロ-β-ラクタマーゼ産生菌が仙台市内の医療機関で探知され、抗菌薬適正使用や公衆衛生上の価値は高いと考えられる。本研究の成果としてあらたに見つかったセフタジジムとセフォペラゾンナトリウム・スルバクタムナトリウムの二剤の感受性結果に注目したスクリーニングは、現場の労力を削減して効率化を図ることのできる有用かつ簡便なスクリーニング手法であり、社会的意義は高いと思われる。