The effects of fluoride on central nervous system from the standpoints of difference of sensitivity

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10612
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58020:Hygiene and public health-related: including laboratory approach
• Research Institution: National Defense Medical College
• Principal Investigator: Tsunoda Masashi 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 衛生学公衆衛生学, 教授 (00271221)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: フッ素 / 神経毒性 / 二世代 / 行動学試験 / ラット / F1動物

Outline of Final Research Achievements

To elucidate the developmental neurotoxicity of rats, pregrant rats were exposed to fluoride (F) at the concentrations of 0 or 200 ppm in the drinking water. After weaning, F1 rats which exposed to F were divided to the two group which administered 0 or 150 ppm of F in their drinking water. The groups were composed of the control, perinatal exposure (FC) and repeated exposure (FF) groups. At the 12 weeks of age, the open field test and the prepulse inhibition (PPI) test were performed. The mean values of body weights in the male FF group were significantly lower than those in the other two groups. The mean % PPI at PP 70 as one of indexes of the PPI test in the male FF group was significantly lower than that of the control. The perinatal exposure to F exposure may have inhibitory effects on the development of F1 rats. The lower % PPI in the F1 rats exposed to F from fetus to the 12 weeks of age may be comparable to the ADHD-like syndrome observed in humans.

Free Research Field

衛生学

Academic Significance and Societal Importance of the Research Achievements

インドや中国において地下水の汚染が大きな問題になっているフッ素について、今まで明らかになっていなかった神経毒性を、胎児期～発達期の感受性の高い成長期に曝露があれば、動物において起こりうることを示唆した。顕著な成長抑制が観察されたことと併せて、フッ素の感受性の高い成長期における曝露にリスクが伴うことを示したことに学術的・社会的意義がある。今後、疫学研究との所見と併せて研究を進めるべき結果となった。