Proteins that help promote neural repair in rehabilitation-aided recovery from brain hemorrhage

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K11366
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Osaka University
• Principal Investigator: Mariko Nishibe 大阪大学, 大学院歯学研究科, 講師 (50638757)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: Stroke / Hemorrhage / Gene expression / Cortex

Outline of Final Research Achievements

We analyzed molecular regulation of the cortical cells following a hemorrhage induced to the ipsilateral striatum. Rehabilitation-specific effects on cortical molecular profile were found to be the upregulation of various promoters, molecules related to blood-vessel repair. We determined that Atf3 protein to be highly expressed in rehabilitation-manner in the cortical layer 5 projection neurons (of which send reciprocal connection with the injured striatum), as well as peri-injury area. The number of pericytes and endothelial cells, that contribute to blood-vessel repair did not significantly change rehabilitation-dependently, though the numbers were significantly higher after hemorrhage. The study suggests that cortical neuro-functional recovery is associated with the upregulation of repair-activity of neurons and molecular levels of blood-vessel repair. We provide evidence of how rehabilitation facilitates the gene/ protein expression of injured neurons in a mouse hemorrhagic model.

Free Research Field

リハビリテーション科学

Academic Significance and Societal Importance of the Research Achievements

レンズ核線条体動脈の出血による被殻出血は、全能出血の40％を占め、上位運動ニューロンと感覚ニューロンに障害をきたし、片麻痺・麻痺側の感覚障害を引き起こす。線条体出血のマウスモデルを使い、脳神経の分子学・生理学データ、また回復期の巧緻運動機能へのリハビリ効果を示した。さらなるメカニズム解明により、脳神経機能回復を完結に近づけるために行うリハビリについて多面的にアプローチする事が可能である。