2021 Fiscal Year Final Research Report
Elucidation of nitrous oxide production mechanisms focusing on the mRNA expression related to nitric oxide production and decomposition
| Project/Area Number |
19K12385
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 64020:Environmental load reduction and remediation-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 亜酸化窒素 / 一酸化窒素 / アンモニア酸化細菌 / mRNA / 転換率モデル |
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| Outline of Final Research Achievements |
The purpose of this research is to elucidate the nitrous oxide production mechanism by ammonia-oxidizing bacteria, especially focusing on the mRNA expression related to nitric oxide production and decomposition. From the previous research, it has been hypothesized that the demand and availability of nitric oxide determines the nitrous oxide production. To verify the hypothesis, the effect of nitric oxide or nitrogen gas bubbling on ammonia oxidation, nitrous oxide production and mRNA expression were investigated. From the results, one part of the hypothesis that the excessive nitic oxide increases the expression of norB mRNA is almost verified. Although further research is required, it is concluded that the demand and availability of nitric oxide could be the critical factor on nitric oxide production. From this research, the basic and essential findings for nitrous oxide production control have been obtained.
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| Free Research Field |
土木環境システム
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| Academic Significance and Societal Importance of the Research Achievements |
脱炭素社会の構築に向けて，エネルギー消費由来でない温室効果ガスの直接的な排出を抑制する技術の開発は喫緊の課題である。本研究は，排水処理において排出される亜酸化窒素の生成抑制技術の開発に貢献するため，生成メカニズムを詳細に検討したものである。アンモニア酸化細菌の状態変化に伴う亜酸化窒素の生成に関連する機能遺伝子同士の転写量の関係が明らかにされ，一酸化窒素の生成・分解とアンモニア酸化細菌の想い（mRNA転写量）に関する仮説を概ね示せたことから，生成抑制技術の開発に極めて重要かつ必須な知見が得られたものと考えている。
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