Development of integrated in vitro monitoring systems for dynamic cell responses

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K12751
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Kindai University
• Principal Investigator: Komori Kikuo 近畿大学, 工学部, 准教授 (60431813)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 電気化学センサ / 細胞アッセイ / カーボンナノ材料 / 生体模倣システム

Outline of Final Research Achievements

We investigated towards the development of label-free cell-based bioassay systems with oxygen-independent electrochemical biosensors. Based on the electrochemical responses obtained here, hepatocytes cultured under an aerobic culture method showed physiologically relevant behaviors of glucose metabolism and gluconeogenesis in the periportal region of liver tissues. In addition, released histamine from a basophilic leukemia cell line, RBL-2H3, was successfully monitored after immunostimulation. The present systems will enable to improve the prediction of human responses.

Free Research Field

生物工学

Academic Significance and Societal Importance of the Research Achievements

動物実験に代わる薬剤や化学物質の有害性や安全性の評価として、ヒト培養細胞を利用したアッセイ法の利用が期待されている。最近の細胞培養手法の高度化により、薬剤に対して生体と同質の応答が得られるようになりつつあるものの、依然として予測精度は低い。この問題解決の1つとして本研究では、好気的な細胞環境条件に加えて、従来の終点計測ではなく、連続計測を組み合わせることで、予測精度の向上を図った。