2022 Fiscal Year Final Research Report
Development of two-photon photoacoustic microscopy with spatial overlap modulation of femtosecond optical pulse trains
| Project/Area Number |
19K12787
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 光音響イメージング / 光音響顕微鏡 / 2光子吸収 / 非線形光学 / 空間重なり変調 / 生体光イメージング |
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| Outline of Final Research Achievements |
Two-photon fluorescence microscopy has been used in the fields of biology and medicine as a method for observing deep parts of living cells and tissues with high spatial resolution. However, the deeper the observation depth is, the worse the image contrast is due to signals from other than the focal point. To overcome this drawback, we have proposed a two-photon photoacoustic microscopy based on spatial overlap modulation using femtosecond optical pulse trains. We have constructed this microscopy system and verified the principle of two-photon photoacoustic wave generation by overlap modulation. In addition, we have clarified that visualization with depth resolution is possible.
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| Free Research Field |
生体医工学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において開発を行った空間重なり変調を用いた2光子光音響顕微鏡により，生体深部を高コントラスト，高空間分解に観察できるようになる．そのため，医療の分野においては，特に皮膚や管空臓器の壁を深部方向に細胞の大きさに匹敵する空間分解能で観察できることが，がんの広がり診断を有効にする．また，生物学の分野においても，ミリメートルを超える深部観察が必要なスフェロイドの評価や脳機能の評価に有効であると考えている．
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