Tissue reconstraction using mouse and chick skin cells

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K12794
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Okayama University of Science
• Principal Investigator: Kataoka Ken 岡山理科大学, 理学部, 教授 (10293317)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 皮膚 / ケラチノサイト / 線維芽細胞 / スフェロイド

Outline of Final Research Achievements

Spheroids which made from isolated mouse keratinocytes and fibroblasts were cultured on the collagen sponge material.Inoculation of mouse fibroblasts into the collagen sponge increased capability of adaptation of the spheroids on mouse skin. The chick skin cells were isolated and inoculated into mouse skin, but there were no difference between them.Mixed culture of mouse and chick cells made no difference in cell biological behavior.

Free Research Field

細胞培養学

Academic Significance and Societal Importance of the Research Achievements

これまでに研究代表者が開発したin vivoマウス皮膚形成モデルを発展させて、in vitroで複数種の細胞を混合して凝集塊すなわちスフェロイドを形成する評価モデルを開発した。このモデルを用いたマウス皮膚細胞とニワトリ皮膚細胞の混合培養では残念がら期待された結果には至らなかったが、評価モデルの開発は研究領域において有用性が高い。